Assessment of the Antimicrobial Activity of Algae Extracts on Bacteria Responsible of External Otitis

External otitis is a diffuse inflammation around the external auditory canal and auricle, which is often occurred by microbial infection. This disease is generally treated using antibiotics, but the frequent occurrence of antibiotic resistance requires the development of new antibiotic agents. In this context, unexplored bioactive natural candidates could be a chance for the production of targeted drugs provided with antimicrobial activity. In this paper, microbial pathogens were isolated from patients with external otitis using ear swabs for over one year, and the antimicrobial activity of the two methanol extracts from selected marine (Dunaliella salina) and freshwater (Pseudokirchneriella subcapitata) microalgae was tested on the isolated pathogens. Totally, 114 bacterial and 11 fungal strains were isolated, of which Staphylococcus spp. (28.8%) and Pseudomonas aeruginosa (P. aeruginosa) (24.8%) were the major pathogens. Only three Staphylococcus aureus (S. aureus) strains and 11 coagulase-negative Staphylococci showed resistance to methicillin. The two algal extracts showed interesting antimicrobial properties, which mostly inhibited the growth of isolated S. aureus, P. aeruginosa, Escherichia coli, and Klebsiella spp. with MICs range of 1.4 × 10⁹ to 2.2 × 10¹⁰ cells/mL. These results suggest that the two algae have potential as resources for the development of antimicrobial agents.     

Growing media for horticultural bedding plants yield under agriculture organic systems

The aim of this experiment was to test on-farm growing after composting the mixture of following legume plants Crotalaria juncea, Cajanus cajan, Mucuna aterrima with the grass Sorghum bicolor. The treatments were compared to commercial growing medium (CGM) and CGM+NPK to produce bedding plants of lettuce, broccoli, kale, and cabbage. The nutrient contents in CGM and CGM+NPK were higher than the values found in the mixture of the legumes plants with the grass. The pH was higher in the growing media from legume plants as well as the electrical conductivity in the combination CroSor and CajSor. The water retention capacity was higher in the treatments MucSor and CGM. The chemical attributes indicated that CroSor, CajSor, MucSor and CGM+NPK were better than CGM. The growing media from the mixture of legume with grass are an affordable alternative to produce bedding plants of lettuce, broccoli, kale and cabbage under organic agriculture system.     

腐殖酸-高岭石胶体对铀(VI)在饱和多孔介质中迁移过程的影响

为探讨可溶性有机质存在条件下黏土矿物胶体对铀（VI）在饱和多孔介质中迁移的影响,选取2种石英砂（细砂和粗砂）分别构建均质构型的石英砂柱,研究了饱和水流条件下腐殖酸存在时高岭石胶体对铀在2种不同粒径石英砂中迁移的影响。结果表明,示踪溶质在2种均质石英砂柱中的穿透曲线相似且分布对称。当在石英砂柱中只通入铀（VI）溶液时,铀（VI）在2种粒径石英砂柱中的穿透量均较小（<25%）,此时大量的铀（VI）通过络合作用吸附在石英砂表面上。当通入溶液中存在腐殖酸和高岭石胶体时,铀（VI）在2种石英砂柱中的穿透量增加,且在600~850 μm石英砂中（58.36%）比250~425 μm石英砂中（42.68%）穿透量更多。这充分表明腐殖酸—高岭石胶体和石英砂粒径是影响铀（VI）在地下环境中迁移的重要因素。     

森林旅游解说规划中的空间、信息与媒体关系模型构建——以天际岭国家森林公园为例

生态文明建设是中华民族永续发展的千年大计,要促进生态、文化和旅游的相互融合。森林旅游解说作为森林旅游过程中必不可少的传播手段,在我国生态文明建设中发挥着重要的作用。以往的文献中,多从游客角度来研究旅游解说规划,空间视角的相关文献甚少,且研究单一。旅游移动性作为旅游的本质属性,从空间视角认识旅游解说规划中的信息与媒体关系将有助于编制科学的旅游解说规划。以湖南天际岭国家森林公园为例,基于实地访谈法与内容分析法,通过对点-线-功能区-公园-区域等5个空间尺度下的80名游客解说需求与媒体偏好进行研究,构建了森林公园解说规划空间尺度、信息需求与媒体偏好概念模型,具有一定的创新性。研究发现,处于不同空间尺度下的游客对解说信息与媒体偏好存在较大的差异性,并指出游客解说信息需求呈现一定的尺度上升趋势,反之则表现不明显。     

TAT-Apoptin基因原核表达载体的构建及其表达鉴定

为得到TAT-Apoptin 融合蛋白,以pMD-19T-Apoptin 为模板PCR 扩增出TATApoptin 基因,构建重组载体pET-32a-TAT-Apoptin,并对TAT-Apoptin 蛋白进行表达。PCR 和测序表明,成功构建重组载体pET-32a-TAT-Apoptin,进一步对其进行蛋白表达,发现该蛋 白能与Apoptin 多抗发生特异性结合。以上结果证明,TAT-Apoptin 基因原核表达载体构建成 功,并能在Rosetta 中进行分泌性表达。     

Study on Secretory Expression and Immunogenicity of PPRV F Gene in Insect Cells

This study was aimed to explore the use of baculovirus expression system to secrete peste des petits ruminants virus (PPRV) F gene protein and use it as subunit vaccine. The gene fragment encoding F protein of PPRV was cloned into the baculovirus pFastBac Ⅰ transfer vector with a honeybee melittin signal peptide.The constructed F-pFastBac was transformed into Escherichia coli DH10Bac,resulting the recombinant baculovirus DNA (F-Bacmid) which was confirmed by blue-white plaque assay and antibiotic resistance selection.The F-Bacmid was then transfected into Sf9 insect cells by the cellfectin transfection reagent.The recombinant F protein was expressed in High Five cells in the serum-free medium.The SDS-PAGE and Western blotting analysis of recombinant protein showed that the protein could be expressed in insect cells and secreted into the culture medium. For the immunogenicity study,the recombinant protein was then inoculated into BALB/c mice, the results showed that the recombinant protein was able to stimulate B cells to produce special antibodies. In conclusions,the recombinant baculovirus expressing F protein of PPRV were successfully constructed.This study applied a basis for the development of PPRV subunit vaccine.     

新媒体对低碳农业发展的影响

介绍了新媒体在低碳农业发展中的应用现状,剖析了新媒体对低碳农业发展的积极影响及消极影响,最后提出了新媒体推动低碳农业发展的对策。     

榕管蓟马防治高效菌株蜡蚧轮枝菌V3450固态发酵基质配方筛选

蜡蚧轮枝菌V3450是榕管蓟马防治的高效菌株,为实现该菌株的放大培养,以盆栽榕树出口使用的主要栽培基质椰糠和单一固态发酵培养产孢量最多的麦麸为主载体,开展该菌株的固态发酵基质一般组合配方和正交组合配方筛选试验。结果表明：一般组合固态发酵基质配方5（椰糠+麦麸+磷酸盐+酵母粉=7.5 g+7.5 g+0.2 g+0.5 g）为最好的初筛配方,蜡蚧轮枝菌V3450菌株固态发酵培养所需基质的4个基本组分因子为麦麸、椰糠、磷酸盐和酵母粉;正交组合固态发酵基质配方8（麦麸:椰糠:磷酸盐:酵母粉=3:2:3:2）为最佳的组合配方,麦麸、椰糠、磷酸盐和酵母粉等4个基本组分因子的最佳质量配比为3:2:3:2。正交组合配方8（麦麸:椰糠:磷酸盐:酵母粉=3:2:3:2）是蜡蚧轮枝菌V3450菌株的最佳固态发酵基质配方,放大培养的真菌孢子对榕管蓟马成虫具有很强的侵染力。     

利用流媒体技术实现机械制造实习网络教学

文章从介绍流媒体技术出发,介绍了流媒体技术与网路视频如何实现有效融合,以提高便重于动手实践的机械制造实习教学效果,希望为广大工程院校提高机械制造实习课程教学效果提高一些技术借鉴.     

人工控温条件下喷播基质对羊草生长特征的影响研究

采用盆栽试验法,以高寒地区常见植物羊草为供试材料,内蒙古阿荣旗至博克图段高速公路示范工程采用的厚层基质为基质原材料,对比研究不同配方基质在15℃、20℃、25℃恒温和室温条件下羊草的出苗及生长情况。结果表明:羊草的出苗率及平均株高均表现为随温度升高而增加的趋势,不同配方在4个温度水平下羊草平均出苗率为配方2 > 客土 > 配方3 > 配方1;平均株高整体上表现为配方2 > 配方1 > 配方3 > 客土,且温度越高,配方2平均株高与其他配方差距越明显。因此,配方2能显著增加植物出苗率及平均株高,是适应温度变化最优的基质配方,即客土与泥炭土的比例为4∶6的基质配方是高寒地区厚层基质护坡最理想的基质。